Studying the expression of proteins in Chrysichthys nigrodigitatus muscle, is essential to understand the biological, physiological and
ecological aspects that may be of advantage in ecotoxicology as a tool for biomonitoring the effects of environmental pollution, as well as
food safety. This study was aimed to accomplish a systematic characterization of the muscle proteome as well as to identify a putative
set of protein biomarkers in C. nigrodigitatus to environmental pollution in Ologe and Badagry lagoons. Fifteen fi sh samples were used
as representative of the population for proteomics analysis. 116 proteins was expressed with 70 up-regulated, 25 down-regulated from
Ologe Lagoon and 30 up-regulated, 17 down-regulated proteins from Badagry Lagoon expressed in the muscle of C. nigrodigitatus in
response to environmental stressors using iTRAQ, while 8 protein spots from 2-D gels, representing 8 proteins with 2 up-regulated and 6
down-regulated, have been identifi ed using MALDI TOF/TOF MS. Pearson correlation revealed signifi cant correlation (p < 0.05) between
environmental variables and protein markers. The investigation revealed that the expressed proteins in the muscle of C. nigrodigitatus
served as a prognostic tool to assess the fi sh health and pollution status, which was observed that the fi sh were physiologically perturbed
by environmental stressors in Ologe and Badagry lagoons.
Ologe and Badagry Lagoons are situated within the Lagos lagoon
complex in Lagos State of Southwestern region of Nigeria. Ologe Lagoon is a freshwater body which opens into the Atlantic
Ocean via the Badagry creeks and the Lagos harbour. Th ese Lagoons
meet several socio-economic needs such as aquaculture, fishing, sand
dredging and drainage across various towns and villages borderingit. By virtue of their position, (with several industries and other
anthropogenic activities around it), have been impacted by partially
treated and untreated industrial effluents.
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